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KMID : 0381120130350030273
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Genes and Genomics
2013 Volume.35 No. 3 p.273 ~ p.288
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Genome-wide expression profiles of contrasting inbred lines of Chinese cabbage, Chiifu and Kenshin, under temperature stress
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Lee Jeong-Yeo
Lim Yong-Pyo
Han Ching-Tack
Nou Ill-Sup
Hur Yoon-Kang
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Abstract
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Abiotic stresses such as extreme temperatures frequently limit plant growth and productivity of major crop species. To identify temperature-stress responsive genes in Brassica rapa ssp. pekinensis, we conducted a comparative transcriptome analysis of two contrasting genotypes, the inbred lines Chiifu and Kenshin, under and heat-shock conditions. Of 23,929 unigenes deposited on a Br24K microarray, 17,603 genes were significantly expressed. Chiifu expression profiles were more prevalent under , and those of Kenshin were more prevalent under heat-shock. Previously characterized genes, with little exception, show similar changes to temperature stress. We found that a relatively large number of genes were expressed in response to temperature stress and genotype, amounting to 24 and 126 genes from and heat-shock conditions, respectively. These genes could be used in molecular marker development for either high or low temperature-resistant Brassica spp. Only ten genes, including TCH4 and CBL1, were up-regulated in both genotypes under all stress treatments, implying possible candidates for broad spectrum, stress-resistant crop production.
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KEYWORD
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Brassica rapa ssp. pekinensis, Light-chilling, Heat-shock, Microarray
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